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YOUR CANNABIS REGULATORY GUIDELINES AND FAST-TRACK TO SOLUTIONS.

As the cannabis industry matures, so do the pathogen testing regulations designed to ensure safe sales and use of Cannabis.

While no national standard for microbial safety of Cannabis exists today, state regulators set identification targets for both pathogen species and concentration levels. Below you can find out exactly what the state your Cannabis lab, grow or processing operation does business in requires and regulates.

In addition, we’ll help you quickly identify the PathogenDx assay designed to drive detection and quantification with industry-lead efficiencies. Start your exploration on the map below—and discover why we’re setting the standard in microbial testing.

(Updated 05/2023)

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Milan Patel, PathogenDx CEO, provides a quick introduction to the state of Cannabis pathogen testing regulations.

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No regulations
Moderate regulations
High regulations
Alabama

Medical Legalized

AOAC Required

Total Enterobac

<1,000 cfu/g (in process) <100 cfu/g (fully processed)

Total Coliform

<1,000 cfu/g

Total Y&M

<10,000 cfu/g (in process) <1,000 cfu/g (fully processed)

Aspergillus

Not detected in 1g

E. coli

Not detected in 1g

Salmonella

Not detected in 1g

STEC

Not detected in 1g (both 1 & 2)

Alaska

View Regulations

Medical + Adult-use Legalized

Salmonella

<1 cfu/g (marijuana/marijuana retail products/water & food based concentrates)

STEC

<1 cfu/g

Aspergillus
(Flavus, Fumigatus, Niger)

<1 cfu/g

Arizona

Medical Regulations
Adult-Use Regulations

Medical + Adult-use Legalized

AOAC Required

Adult Use

E. coli

Less than 100 cfu/g (adult & medical)

Salmonella

Not detected in 1g (adult & medical)

Aspergillus

Not detected in 1g (inhalable) (medical products only)

Medical Use

E.Coli

100 CFU/g

Salmonella

Detectable in 1 gram

Aspergillus
(Flavus, Fumigatus, Niger, Terreus)

Inhalable: Detectable in 1 gram

Arkansas

View Regulations

Medical Legalized

Total Coliform

If detected, must test for E. coli (usable marijuana, extracts & edibles)

E. coli

If more than 100 cfu/g, sample fails (usable marijuana, extracts & edibles)

California

View Regulations

Medical + Adult-use Legalized

 

Berkeley, California

View Regulations

Medical + Adult-use Legalized

Salmonella spp.

Not detected in 1 gram (flower & oral)

STEC

Not detected in 1 gram (flower & oral)

Aspergillus
(Flavus, Fumigatus, Niger, Terreus)

Not detected in 1 gram (flower & oral)

Berkeley, California

Salmonella

0 CFU

E.Coli

0 CFU

Total Aerobic (APC)

<100,000 CFUs

Total Yeast Mold

APC < 10 CFUs

Pseudomonas

≤ 1,000 CFUs

Total Coliform

≤ 1,000 CFUs

Colorado

View Regulations

Medical + Adult-use Legalized

AOAC Required

Regulated Marijuana Flower and trim; Regulated Marijuana Products (other than Audited Product); Water-Based, Heat/Pressure-Based, and Food-Based Medical Marijuana Concentrate; Water-Based, Heat/Pressure-Based, and Food-Based Retail Marijuana Concentrate; Industrial Hemp Products; Pressurized Metered Dose Inhalers; Vaporizer Delivery Device

Salmonella

< 1 Colony Forming Unit CFU

STEC

< 1 Colony Forming Unit CFU

Total Yeast Mold

< 104 Colony Forming Unit CFU

Administration by metered dose nasal spray or vaginal administration

Total Yeast Mold

≤102 cfu/ml or ≤102 cfu/g

Total Aerobic

≤102 cfu/ml or ≤102 cfu/g

Staphylococcus aureus

Absent in 1 ml or 1 g

Pseudomonas aeruginosa

Absent in 1 ml or 1 g

Bile Tolerant Gram Negative Bacteria

Absent in 1 ml or 1 g
(Only tested for administration by metered dose nasal spray products)

Candida Albicans

Absent in 1 ml or 1 g
(Only tested for vaginal administration products)

Rectal administration products

Total Yeast Mold

≤102 cfu/ml or ≤102 cfu/g

Total Aerobic

≤103 cfu/ml or ≤103 cfu/g

Connecticut

View Regulations
USP Regulations

Medical + Adult-use Legalized

Salmonella

Not detected in 10g

E.coli

Not detected in 10g

Total Aerobic

<105 Colony Forming Unit CFU

Total Yeast Mold

<103 Colony Forming Unit CFU

Bile Tolerant Gram Negative Bacteria

<103 Colony Forming Unit CFU

Pseudomonas

Absense in 1g or 1ml

Delaware

View Regulations

Medical Legalized

E.coli

N/A

Pseudomonas aeruginosa

N/A

Aspergillus
● A. flavus
● A. fumigatus
● A. niger
● A. terreus

N/A

Total Aerobic

N/A

Total Yeast Mold

N/A

Total Coliform

N/A

Staphylococcus Aureus

N/A

Washington DC

View Regulations

Medical + Adult-use Legalized

E.coli

< 100 CFU per gram

Salmonella spp.

0 CFU per gram

Total Aerobic

100,000 CFU per gram

Total Yeast and Mold

10,000 CFU per gram

Florida

View Regulations

Medical Legalized

Usable Whole Flower Marijuana, Derivative Products, and Edibles

Salmonella

<1 CFU per gram

STEC

<1 CFU per gram

Aspergillus
● A. flavus
● A. fumigatus
● A. niger
● A. terreus

<1 CFU per gram

Total Yeast and Mold

<100,000 CFU per gram

Non-Oral Transmucosal Products

Total Yeast and Mold

<10 CFU per gram

Staphylococcus aureus

<1 CFU per gram

Bile Tolerant Gram Negative Bacteria

<1 CFU per gram

Total Aerobic Microbial Count

<100 CFU per gram

Georgia

Not Legal

Medical use and Adult-use is not permitted
except for CBD oil for medical use.

Guam

View Regulations
(proposed, not finalized as of 12/2021)

Medical + Adult-use Legalized

Proposed Testing Regulations

Hawaii

View Regulations

Medical Legalized

Unprocessed and processed materials

Salmonella

Not detected in 1 gram

E.coli

Not detected in 1 gram

Total Aerobic

<100,000 CFU per gram

Total Yeast Mold

<20,000 CFU per gram

Bile Tolerant Gram Negative Bacteria

<1,000 CFU per gram

Total Coliform

<1,000 CFU per gram

Aspergillus
● A. flavus
● A. fumigatus
● A. niger

<1 CFU per gram

Carbon dioxide and solvent based extracts

Salmonella

Not detected in 1 gram

E.coli

Not detected in 1 gram

Total Aerobic

<10,000 CFU per gram

Total Yeast Mold

<100 CFU per gram

Bile Tolerant Gram Negative

<100 CFU per gram

Total Coliform

<100 CFU per gram

Aspergillus
● A. flavus
● A. fumigatus
● A. niger

<1 CFU per gram

Illinois

View Regulations

Medical + Adult-use Legalized

Salmonella

Not detected in 1 gram

STEC

Not detected in 1 gram

Total Aerobic

<104 CFU per gram

Total Aerobic

<103 CFU per gram

Bile Tolerant Gram Negative Bacteria

<102 CFU per gram

Total Coliform

<102 CFU per gram

Idaho

Not Legal

No testing regulations

Indiana

Not Legal

No testing regulations

Iowa

View Regulations

Medical Legalized

Consumable Products

Salmonella

Not detected in 1 gram

STEC

Not detected in 1 gram

Total Aerobic

<103 CFU per gram

Total Yeast Mold

<102 CFU per gram

Inhalable Products

Salmonella

Not detected in 1 gram

STEC

Not detected in 1 gram

Total Aerobic

<102 CFU per gram

Total Yeast Mold

<101 CFU per gram

Aspergillus
● A. flavus
● A. fumigatus
● A. niger
● A. terreus

<102 CFU per gram

Non-consumable Products (Topical, Suppositories)

Total Aerobic

<103 CFU per gram

Total Yeast Mold

<102 CFU per gram

Kansas

Not Legal

No testing regulations

Kentucky

Not Legal

No testing regulations

Louisiana

View Regulations

Medical Legalized

Salmonella

<1 CFU/gram

STEC

<1 CFU/gram

Total Yeast Mold

<10,000 CFU/gram

Maine

View Regulations

Medical + Adult-use Legalized

Unprocessed and Processed Plant Material

Salmonella spp.

<1 CFU/gram

E. coli

<1 CFU/gram

Total Aerobic

<105 CFU per gram

Total Yeast and Mold

<104 CFU per gram

Enterobacteriaceae

<103 CFU per gram

Total Coliform

<103 CFU per gram

CO2 and Solvent-Based Extracts

Salmonella spp.

<1 CFU/gram

E. coli

<1 CFU/gram

Total Aerobic

<104 CFU per gram

Total Yeast and Mold

<103 CFU per gram

Enterobacteriaceae

<102 CFU per gram

Total Coliform

<102 CFU per gram

Maryland

View Regulations

Medical Legalized

Flower and Processed Products

Salmonella

None Detected

E.coli

<1 CFU per gram

Total Aerobic

<100,000 CFU per gram

Total Yeast Mold

<100,000 CFU per gram

Edibles Products

Total Coliforms

<10 CFU per gram

STEC

None Detected

Salmonella, spp

None Detected

L. monocytogenes

None Detected

Massachusetts

View Regulations

Medical + Adult-use Legalized

Salmonella

Not Detected in 1 g

STEC

Not Detected in 1 g

Total Aerobic

<105 CFU per gram

Total Yeast Mold

<104 CFU per gram

Bile Tolerant Gram Negative Bacteria

<103 CFU per gram

Total Coliform

<103 CFU per gram

Michigan

View Regulations

Medical + Adult-use Legalized

Bud, shake, trim, kief, and inhalable compound concentrate products (medical and adult-use)

Salmonella

Not Detected in 1 g

STEC

Not Detected in 1 g

Total Yeast Mold

<10,000 CFU/g (medical) <100,000 CFU/g (adult-use)

Total Coliform

<1,000 CFU/g

Aspergillus
A. flavus
A. fumigatus
A. niger
A. terreus

If Aspergillus spp. is detected, the sample fails.

Infused Products

Salmonella

Not Detected in 1 g

STEC

Not Detected in 1 g

Total Yeast Mold

<10,000 CFU/g

Total Coliform

<100 CFU/g

Aspergillus
A. flavus
A. fumigatus
A. niger
A. terreus

If Aspergillus spp. is detected, the sample fails

Non-CO2 and Non-Solvent based extracts

Salmonella

Not Detected in 1 g

STEC

Not Detected in 1 g

Total Yeast Mold

<1,000 CFU/g

Total Coliform

<100 CFU/g

Aspergillus
A. flavus
A. fumigatus
A. niger
A. terreus

If Aspergillus spp. is detected, the sample fails

Minnesota

View Regulations

Medical Legalized

Salmonella

Not detected in 1 gram

E.coli

<7 CFU/g

Total Aerobic

<100,000 CFU/g

Total Yeast Mold

<1,000 CFU/g

Bile Tolerant Gram Negative Bacteria

<150 CFU/g

Mississippi

View Regulations

Medical Legalized

Inhalable cannabis and cannabis products

Salmonella spp.

is not detected in 1 gram

STEC

is not detected in 1 gram

Aspergillus
● A. flavus
● A. fumigatus
● A. niger
● A. terreus

is not detected in 1 gram

Non-inhalable cannabis and cannabis products

Salmonella spp.

is not detected in 1 gram

STEC

is not detected in 1 gram

Missouri

View Regulations

Medical Legalized

Salmonella

Not Detected in 1 g

STEC

Not Detected in 1 g

Aspergillus
● A. flavus
● A. fumigatus
● A. niger
● A. terreus

Not Detected in 1 g

Montana

View Regulations

Medical + Adult-use Legalized

Salmonella

Not Detected in 1 g

E.coli

Not Detected in 1 g

Total Yeast Mold

<10,000 CFU/g

Nebraska

Not Legal

No testing regulations

Nevada

View Regulations

Medical + Adult-use Legalized

Usable marijuana & crude collected resins, as received, and wet marijuana, as received, which is destined for extraction

Salmonella

Not Detected in 1 g

STEC

Not Detected in 1 g

Total Enterobacteriaceae

< 1,000 colony forming units per gram

Total Coliform

< 1,000 colony forming units per gram

Aspergillus
● A. flavus
● A. fumigatus
● A. niger
● A. terreus

Not Detected in 1 g

Total Yeast and Mold

< 10,000 colony forming units per gram

Extract of marijuana (nonsolvent) like hashish, bubble hash, infused dairy butter, mixtures of extracted products or oils or fats derived from natural sources, including concentrated marijuana extracted with CO2, Extract of marijuana (solvent-based) made with any approved solvent, including concentrated marijuana extracted by means other than with CO2

Salmonella

Total Yeast and Mold

STEC

Total Yeast and Mold

Total Enterobacteriaceae

< 100 colony forming units per gram

Aspergillus
● A. flavus
● A. fumigatus
● A. niger
● A. terreus

Total Yeast and Mold

Total Yeast and Mold

< 1,000 colony forming units per gram

Edible marijuana products and Liquid marijuana products, including, without limitation, soda or tonic, including a product which contains concentrated marijuana

Total Enterobacteriaceae

Not Detected in 1 g

Salmonella

Not Detected in 1 g

STEC

Not Detected in 1 g

Total aerobic count

< 100,000 colony forming units per gram

New Hampshire

View Regulations

Medical Legalized

Harvested Cannabis

STEC

Not Detected in 1 g

Total Aerobic

< 105 CFU/g

Total Yeast Mold

< 104 CFU/g

Bile Tolerant Gram Negative Bacteria

< 103 CFU/g

Total Coliform

< 103 CFU/g

Salmonella

Not Detected in 1 g

Cannabis Concentrate

STEC

Not Detected in 1 g

Total Aerobic

< 104 CFU/g

Total Yeast Mold

< 103 CFU/g

Bile Tolerant Gram Negative

< 102 CFU/g

Total Coliform

< 102 CFU/g

Salmonella

Not Detected in 1 g

New Jersey

Medical + Adult-use Legalized

No testing regulations

New Mexico

View Regulations

Medical Legalized
(Adult-use Legalized. Testing Rules not promulgated as of 8/26/21)

Chopped or Powdered Botanicals (Dried Usable Cannabis Not Extracted)

Total Aerobic Microbial Count

>100,000 cfu/g or cfu/mL

Total Combined Yeast & Mold Count

>1,000 cfu/g or cfu/mL

Bile-tolerant Gram-negative Bacteria

>1,000 cfu/g or cfu/mL

Absence of Salmonella spp. & E. coli

Absent In 10 grams cfu/g or cfu/mL

Total Coliforms Count

>1,000 cfu/g or cfu/mL

Powdered Botanical Extracts (Extracted or Processed Cannabis Product i.e. hash, bubble hash, rosin, kief)

Total Aerobic Microbial Count

>1,000 cfu/g or cfu/mL

Total Combined Yeast & Mold Count

>1,000 cfu/g or cfu/mL

Bile-tolerant Gram-negative Bacteria

>1,000 cfu/g or cfu/mL

Absence of Salmonella spp. & E. coli

Absent In 10 grams cfu/g or cfu/mL

Total Coliforms Count

>1,000 cfu/g or cfu/mL

Tinctures (Solutions of Cannabis in Alcohol) & Fluid extracts (An alcoholic liquid extract made by percolation of Cannabis so that 1 mL of the fluid extract represents 1 g of the Cannabis)

Total Aerobic Microbial Count

>10,000 cfu/g or cfu/mL

Total Combined Yeast & Mold Count

>1,000 cfu/g or cfu/mL

Infusions (Solutions of Cannabis in water) & Decoctions (Solutions of Cannabis derived by boiling in water for at least 15 minutes)

Total Aerobic Microbial Count

>100 cfu/g or cfu/mL

Total Combined Yeast & Mold Count

>100 cfu/g or cfu/mL

Nutritional Supplements with Botanicals & Botanicals to be treated with boiling water before use (Dried Cannabis to which boiling water is added immediately prior to consumption)

Total Aerobic Microbial Count

Total Aerobic Microbial Count

Total Combined Yeast & Mold Count

>1,000 cfu/g or cfu/mL

Absence of Salmonella spp. & E. coli

Absent In 10 grams cfu/g or cfu/mL

Nutritional products with other highly refined ingredients (Edibles)

Total Aerobic Microbial Count

>1,000 cfu/g or cfu/mL

Total Combined Yeast & Mold Count

>100 cfu/g or cfu/mL

Absence of E.coli

Absent In 10 grams cfu/g or cfu/mL

New York

View Regulations

Medical + Adult-use Legalized

E.coli

Pseudomonas (Vaporized products)

Salmonella

Enterococcus species

Bile Tolerant Gram Negative Bacteria

Clostridium botulinum

Aspergillus species

Mucor species

Penicillium species

Thermophilic Actinomycetes species

North Carolina

Not Legal

No testing regulations

North Dakota

View Regulations

Medical Legalized

Dried Leaves or Flowers and Medical Cannabinoid Products

Salmonella

Not detected in one gram

STEC

Not detected in one gram

Total Aerobic

105 CFU/g

Total Yeast Mold

104 CFU/g

Bile Tolerant Gram Negative Bacteria

103 CFU/g

Total Coliform

103 CFU/g

Cannabis Concentrates

Salmonella

Not detected in one gram

STEC

Not detected in one gram

Total Aerobic

104 CFU/g

Total Yeast Mold

103 CFU/g

Bile Tolerant Gram Negative Bacteria

102 CFU/g

Total Coliform

102 CFU/g

Ohio

View Regulations

Medical Legalized

Dried, Cured Plant Material (cfu/g)

Salmonella spp.

Not detected in 1 gram

STEC

Not detected in 1 gram

Total Aerobic Bacteria

≤100,000

Total Yeast and Mold

≤10,000

Bile-tolerant Gram-negative Bacteria

≤1,000

Total Coliforms

≤1,000

Oklahoma

View Regulations

Medical Legalized

Medical Marijuana; Medical Marijuana Products, including medical marijuana concentrates but not including rectal administration products, vaginal administration products, pressurized metered dose inhaler products, and metered dose nasal spray products

STEC

<1 CFU per gram

Salmonella

<1 CFU per gram

Aspergillus
● A. flavus
● A. fumigatus
● A. niger
● A. terreus

<1 CFU per gram

Total Yeast and Mold

<104 CFU per gram

Metered dose nasal spray products and pressurized metered dose inhaler products

Total Yeast and Mold

<101 CFU per gram or milliliter

Total Aerobic Microbial

<102 CFU per gram or milliliter

Staphylococcus aureus

<1 CFU per gram or milliliter

Bile tolerant gram negative bacteria

<1 CFU per gram or milliliter

Rectal Administration products

Total Yeast and Mold

<102 CFU per gram or milliliter

Total Aerobic

<103 CFU per gram or milliliter

Vaginal Administration products

Total Yeast and Mold

<101 CFU per gram or milliliter

Total Aerobic Microbial

<102 CFU per gram or milliliter

Staphylococcus aureus

<1 CFU per gram or milliliter

Pseudomonas aeruginosa

<1 CFU per gram or milliliter

Candida albicans

<1 CFU per gram or milliliter

Oregon

View Regulations

Medical + Adult-use Legalized

Total Coliform

If detected, the sample must be tested for E.Coli

E.Coli

<100 CFU per gram

Pennsylvania

View Regulations

Medical + Adult-use Legalized

Salmonella

Not detected in one gram

E.Coli

Not detected in one gram

Total Aerobic

<10,000 CFU/g Harvest Lot <10,000 CFU/g Process Lot (including flower at product testing)

Total Yeast Mold

<10,000 CFU/g Harvest Lot < 1,000 CFU/g Process Lot < 10,000 CFU/g Final processed flower at product testing)

Bile Tolerant Gram Negative

<1,000 CFU/g Harvest Lot <100 CFU/g Process Lot <1,000 CFU/g Final flower at product testing)

Puerto Rico

No testing regulations

Rhode Island

View Regulations

Medical Legalized

Salmonella

Not detected in one gram

STEC

Not detected in one gram

Total Aerobic

<100,000 CFU/g

Total Yeast Mold

<10,000 CFU/g

Bile Tolerant Gram Negative Bacteria

<1,000 CFU/g

South Carolina

Not Legal

No testing regulations

South Dakota

View Regulations

Medical Legalized

Cannabis, inhalable cannabis products, and any other cannabis product

Salmonella

<1 CFU per gram

STEC

<1 CFU per gram

Aspergillus
● A. flavus
● A. fumigatus
● A. niger
● A. terreus

<1 CFU per gram

Total Yeast and Mold

<10,000 CFU per gram

Total Aerobic Bacteria

<100,000 CFU per gram

Bile Tolerant Gram Negative Bacteria

<1,000 CFU per gram

Tennessee

Not Legal

No testing regulations

Texas

Medical use of CBD oil

No testing regulations

Utah

Total Aerobic

<105 CFU per gram

Total Yeast Mold

<104 CFU per gram

Bile Tolerant Gram Negative

<103 CFU per gram

Salmonella

Absent in 10 grams

E.Coli

Absent in 10 grams

Vermont

View Regulations

Medical + Adult-use Legalized

Action limits for products and infused-products

Total Aerobic

1,000 Colony Forming Unit CFU/g or ml

Total Yeast Mold

100 Colony Forming Unit CFU/g or ml

Action limits for concentrates

Total Aerobic

10,000 Colony Forming Unit CFU/g or ml

Total Yeast Mold

1,000 Colony Forming Unit CFU/g or ml

Action limits for trim flower

Total Aerobic

100,000 Colony Forming Unit CFU/g or ml

Total Yeast Mold

10,000 Colony Forming Unit CFU/g or ml

Virginia

Medical + Adult-use Legalized

No testing regulations

Washington

View Regulations

Medical + Adult-use Legalized

Unprocessed Plant Material

Salmonella spp.

Not detected in one gram

STEC

Not detected in one gram

Bile Tolerant Gram Negative Bacteria

≤104 Colony Forming Unit CFU/g

Extracted or Processed Botanical Product

Salmonella spp.

Not detected in one gram

STEC

Not detected in one gram

Bile Tolerant Gram Negative Bacteria

≤103 Colony Forming Unit CFU/g

West Virginia

View Regulations

Medical Legalized

Total Aerobic

Total Yeast and Mold

Pseudomonas aeruginosa

Aspergillus spp.

Wisconsin

Not Legal

No testing regulations

Wyoming

Not Legal

No testing regulations